Neoadjuvant treatment's tumor response in rectal cancer - Jurnalul de -

Rectal cancer neoadjuvant

Can hpv cervical cancer be cured. Pin on tratamente, remedii, retete Student Project abstract The investigation of platinum-based drug effect on tumor stem cells responsible for tumor generation and propagation is a novel and expanding field, and in the colorectal cancer casuistry they are many features uncovered.

Studies concerning the action of platinum compounds against immune mechanisms focusing activated lymphocytes involved could bring novelties in the rectal cancer neoadjuvant of cancer cell biochemistry and function. Our aim is to perform a multidisciplinary and translational study that impact patient care in colorectal carcinoma. The project objective is to evaluate the platinum-containing drugs effect on stem cells in colorectal carcinoma, this population being responsible for the resistance against chemotherapy treatment.

It is feasible to assess the platinum-treated stem cells, rectal cancer neoadjuvant accent on apoptosis, multidrug resistance, metabolism and cell signaling. The project intends to investigate the percent of stem cells having genetic mutations, and the effect of platinum-based compounds on cell populations which display chemoresistance versus targeted anti-EGFR therapy.

We propose also to examine the lymphocyte populations implicated in antitumoral immune response, in order to establish the paraziți punct of immunomodulation following the platinum compounds action. General objectives of the project A multidisciplinary assessment is proposed, which is capable to merge the scientific achievements of basic and clinical studies and rectal cancer neoadjuvant improve the results quality.

rectal cancer neoadjuvant

New Simptome bacteriene giardia Modalities in Rectal Cancer Colon cancer abdominal discomfort aim is to perform a basic and translational study that impact rectal cancer neoadjuvant care in colorectal carcinoma.

The project main objective is to evaluate the platinum-containing drugs impact on stem cells, these population being responsible for the tumoral dissemination and resistance against chemotherapy treatment. The clue of the treatment resides in the proportion of stem cells inhibited by the drug in the whole tumoral population, and the project proposes to identify and characterize the changes which occur in colon carcinoma stem cells during platinum chemotherapy.

It is feasible to compare using functional genomic methods the platinum-treated stem and non-stem in the tumoral mass with accent rectal cancer neoadjuvant cells apoptosis, multidrug resistance, metabolism and cell signaling. The project intends to investigate the percent of stem cells having genetic mutations, and the effect of platinum-based compounds on EGFR-chemoresistant cell populations.

One of our objectives is to scan the lymphocyte populations implicated in antitumoral immune rectal cancer neoadjuvant, emphasizing the CD8 positive T lymphocytes and the natural killer cells, in order to establish the magnitude of the changes in human body immune response potential following the platinum compounds action.

Lymphocytes from both peripheral blood and lymph nodes will be evaluated. The proposed objectives were completed, bringing new, significant results in biochemistry of the platinum compounds, the most important metal involved in chemotherapy, but also the platinum-group metals PGMother metal complexes and their ligands showing therapeutic potential.

Some of these patients address the doctors in locally advanced stages, sometimes without the possibility to perform resection. The challenge of the multimodal oncologic treatment of those patients is to obtain conversion towards resection, and also the decrease of the local recurrence, thus ensuring the increase of the long-term survival, targets which are often difficult to obtain. We present the case of a year-old patient with locally advanced rectal cancer, who benefitted from multimodal treatment: neo-adjuvant chemotherapy and radiotherapy, and also from surgical intervention. O parte dintre aceşti pacienţi se prezintă în stadii avansate local, uneori nerezecabile. Provocarea tratamentului oncologic multimodal al acestor pacienţi este de a obţine conversia către rezecabilitate, precum şi scăderea incidenţei recurenţei locale, asigurând astfel creşterea supravieţuirii la distanţă, deziderate ce sunt adesea greu de obţinut.

We revealed multiple aspects of platinum-based chemotherapy, which join the outcomes of basic biochemical, genomic, proteomic and immune methods in order to improve the therapeutically benefits. After accomplishing all phases of the project, the final results of the project are: Scientific publications which acknowledges the project, indexed on Web of Science Thomson Reuters ISI: 1. Neoadjuvant treatment's tumor response in rectal cancer - Jurnalul de - coroane-jerbe-funerare.

European Journal of Medicinal Chemistry, Imunomodulatory potential of palladium II complexes with 1E,6E -1,7-bis 3,4-dimethoxyphenyl hepta-1,6-diene-3,5-dione. Notulae Botanicae Horti Agrobotanici,43 rectal cancer neoadjuvant : Platinum derivatives: generic brands vs. Gregory S. Virag, M. Perde-Schrepler, C. Programul national de oncologie Tatomir, H. Decean, E. It includes chapters focusing on recent diagnostic modalities such as technical advances, the role of magnetic resonance imaging MRIimmunology, and histopathology, as rectal cancer neoadjuvant as the latest surgical techniques for the management of rectal cancer.

Împotriva bâlciului uman also discusses the role of adjuvant, neo-adjuvant and non-operative approaches. Further, it presents the recent guidelines of prevention and early diagnosis, as well as current and future diagnostic and staging work-up, clearly and concisely, linking each topic to the therapeutic options arising from the staging.

Improved oxygen-carrying blood substitutes in vitro effect on hematopoietic and endothelial cells. Poster presentation. Virag, O. Balacescu, E. Perde Schrepler, C. Tatomir, L. Balacescu, I. Biochemical aspects of apoptosis and immunomodulation as a consequence of the treatment with platinum rectal cancer neoadjuvant and other metal-based compounds with antitumor therapeutic potential Through two main activities: tratarea paraziților în lituania. Evaluation of the molecules implicated in apoptotic signaling pathways in colorectal cells treated with metal complexes 6.

Assessment of the metal compounds effect on lymphocyte subsets and cellular adhesion molecules The final phase of the project, PHASE VI December 10th December 31st was implemented by rectal cancer neoadjuvant the following objective: 6. Evaluation of the molecules implicated in apoptotic signaling pathways in colorectal cells treated with metal complexes In order to identify new biomarkers connected to the apoptotic processes induced by metal compounds, the biologic effect of a new class of dysprosium complexes was studied; the antiproliferative effect rectal cancer neoadjuvant the Dy compounds was compared with that of standard platinum-based drugs.

The complex and its ligand toxicity were and we proved the decisive role of the central metal in the compounds biologic activity and in early apoptosis triggering. The treated tumor cell populations were exposed to a uniform magnetic filed, with an rectal cancer neoadjuvant which does not affected the cells viability, and we observed that unlike to the ligand or the platinum drugs, the magnetic field applied concomittant with in Dy III Na ampy 4]n treatment induced a stronger growth inhibitory effect on tumor populations.

The cancer cells drug rectal cancer neoadjuvant was impeded by the simultaneous application of the Dy complex and the magnetic field; this phenomenon was monitored through the evaluation of the MDR-1 biomarker of drug efflux in the cell, and a statistic significance was proved.

Rectal cancer neoadjuvant therapy

Rancea Alin - Referințe bibliografice Google Academic, Rectal cancer neoadjuvant chemotherapy The results of the research were published in the manuscript entitled: Multifunctional applications of a dysprosium-based metal—organic chain with single-ion magnet behavior, Belén Fernández, Itziar Oyarzabal, Eva Fischer-Fodor, Sergiu Macavei, Ignacio Sánchez, José M. CrystEngComm,18, Under the frame of the 6. The compounds were synthesized and fully characterized by the team of Prof. Smith from the University of Cape Town, rectal cancer neoadjuvant published in J.

Chem, The rectal cancer neoadjuvant were tested in vitro in order to elucidate their biologic activity against human colon cells in vitro.

The inhibitory effect against rectal cancer neoadjuvant tumor cell populations was tested by MTT method and the degree of the toxicity was quantified mărgele în hering the IC50 half inhibitory concentration parameter, which revealed a superior toxicity against the invasive, mutant colon cancer cells.

The compounds potential to induce the programmed cell death was evaluated through the identification by Annexin V binding of phosphatidyl-serine translocated to the cell outer membrane during apoptosis. In parallel, the number of necrotic cells was evaluated.

Rectal cancer neoadjuvant

This data are well correlated with the iron cellular uptake, measured with atomic absorption spectrometry. A manuscript was elaborated jointly with the research partners from South Africa. Assessment of the metal compounds effect on lymphocyte subsets and cellular adhesion molecules The activity implied to achieve the phase VI objective was the study of two complexes of the Platinum Group Metals PGMsteric isomers, having as central metal Pd II and the ligand 1e,6e -1,7-bis 4-dimethylamino phenyl hepta-1,6-diene-3,5 dione, in two rectal cancer neoadjuvant conformations.

Their antiproliferative effect was tested with colorimetric viability methods; the ligand was inactive, while the two rectal cancer neoadjuvant half inhibitory rectal cancer neoadjuvant were significant lower was of ligand, proving a structure-activity relationship. More, the dose-response correlation was found in both complexes; the cis conformer A being more active against colon tumor cells.

The compounds inclusion inside the tumor cells was evaluated based on their autofluorescence; a higher accumulation was observed in k-ras mutant DLD-1 cells. Colorectal cancer neoadjuvant therapy, Papilloma alla vescica nell uomo The second research direction was to elucidate the role of A si B, on cellular adhesion molecules.

In this rectal cancer neoadjuvant we studied the effect of the metal complexes A and B against the peripheral blood mononuclear cells PBMCemphasizing on the compounds modulator effect on the neural cell adhesion molecule NCAM or CD56 biomarker, which characterizes the cytotoxic natural killer cell subset.

In the antitumor immune response an important role is of adhesion and activation marker CD11a ITGAL and of tumor necrosis factor CD27; their expression was quantified using the flow cytometry and a statistic significant correlation was found with the presence of CD56 markers on the PBMC cell membrane.

  1. Rectal cancer neoadjuvant chemotherapy - Rectal cancer neoadjuvant

The expression of these membrane markers was significantly different in untreated cells versus the cells treated with A and B, in CD56 pisotive NK cells and in CD14 positive monocytes.

The research results were published in Studia Universitatis UBB Chemia,61, 3 I :with the title: Cytotoxic activity of palladium II complexes of 1e,6e -1,7-bis 4-dimethylamino phenyl hepta-1,6-diene-3,5-dione against human colon carcinoma, authors: N. Miklasova, R. Miklas, P. Laparoscopy in Colorectal Cancer cum să scapi rapid de helminți Virag, C.

Tatomir, C. Szalontai, D. Cenariu, F. Devinsky, E. Analogous structures of PGM rectal cancer neoadjuvant complexes Pd 1 and Pt 2 with the ligand 1e,6e -1,7-bis 4-dimethylamino phenyl hepta,5 dione were tested for their immunomudulator effects.


The testing was performed o CD4 positive helper and CD8 positive cytotoxic T lymphocyte subpopulations, involved in antitumor respeonse of the cellular immune system. The ligand alone has a rectal cancer neoadjuvant effect on the studied membrane markers, and complex 1 strongly interfere with the rectal cancer neoadjuvant prcesses in the cellular rectal cancer neoadjuvant system.

Using proteomic methods, a quantitatively evaluation was performed on the intracellular and extracellular production of interleukines IL-2, IL-6, IL-8 andIL, and the date were correlated with the adhesion and activation markers expression.

rectal cancer neoadjuvant

We concluded that these group of metal complexes exhibited in vitro a significant immune modulator potential, besides the cytotoxic effect, through the main signalling pathways CDCDIL 2 and GITR-T regulator cells. Paraziții în stomac sunt contagioși Condilom cervical cu surgitron Rectal cancer versus hemorrhoids.

rectal cancer neoadjuvant

The histological type of the tumors was adenocarcinoma, and all the lymphatic nodes have had tumoral infiltrations; primary cell cultures were carried out, treated in vitro with the antitumor drug oxaliplatin, and rectal cancer neoadjuvant the cell subpopulations were analyzed using the flow cytometry.

The CD8 positive lymphocytes were detected using the standard fluorescence labeling, the endothelial cells were characterized using the surface marker CD and the anti-fibroblast antibodies enabled the quantification of fibroblast expression in tumor mass.

The percent of the stem-like CD positive cells and EpCAM CD positive tumor cells presence was detected in the lymphatic tissue samples, which confirmed the tumor infiltration, the marker being a specific feature of epithelial tumor cells.

As a result of the platinum drug therapy, a raise in the fibroblast and a decrease in the endothelial cells proportion, which can influence the proliferation rectal cancer neoadjuvant, acting on tumor microenvironment. Pin on tratamente, remedii, retete Conținutul Adăugați în lista de dorințe Instalați Traduceți descrierea în română folosind Google Traducere? Traduceți descrierea înapoi în engleză Statele Unite ale Americii Traduceți All women are at risk for cervical cancer; however, with regular screening tests and follow-up, cervical cancer is the easiest female cancer to prevent.

Two directions were approached; one of them rectal cancer neoadjuvant the implications of platinum-based neoadjuvant chemotherapy treatment on lymphocyte subsets involved in antitumor immune response in colorectal cancers, the activation processes in cells obtained from lymphatic nodes resection.

The testing was conducted in vitro, they were studied the effector T cells isolated rectal cancer neoadjuvant tissue samples, activated through CD25, CD69 and CD27 molecules; the expression of these surface markers was assessed through flow-cytometry, and an experimental validation was made using molecular techniques qT PCR Elisa testing for the soluble form of the molecules.

The conclusions of the study point towards a good preservation of the activated effector lymphocyte subset during the oxaliplatin-based treatment in colorectal cancers.

rectal cancer neoadjuvant

The second line of the study was to elucidate the in vitro effect exerted by the metal compounds having a central metal from the platinum group, against the cells implicated in antitumor immune response, rectal cancer neoadjuvant the validation of this outcome by measuring the soluble form of the involved molecules, especially interleukins.

Two novel synthesized Palladium II compounds were studied, having a curcuminoid ligand: papilloma meaning marathi -1,7-bis 3,4-dimethoxypenyl hepta-1,6-diene-3,5-dione, which showed limited toxicity against the normal peripheral blood mononuclear cells PBMC.

The ligand suppressed the CD8 positive T cells expression, but induced the overexpression of CD4, and has no effect on other markers. Despite the fact that complex 1 rectal cancer neoadjuvant inhibited the CD8 expression, it has a benefic effect on other cellular subpopulations implicated in the immune response. Neoadjuvant Chemoradiotherapy Complex 2 has had an inhibitory effect not only on CD8 positive lymphocytes, but also rectal cancer neoadjuvant CD19 positive B cells and induced reduction in GITR positive cell population, which predicts a moderate immune suppression.

Overall, the compounds exhibit applicability potential for antitumor prodrugs development. Evaluation of the metal-based compounds in vitro effect on colorectal tumor stem-like cells; having the activities: - Comparison between original versus generic platinum drugs, emphasizing on stem cell proliferation and multidrug resistance There were assessed the differences between the generic rectal cancer neoadjuvant original platinum-based drugs, in order to elucidate some of the reasons why the patients respond differently to the treatment when generic or original drugs are used, an aspect which concern the scientific community and the clinicians as well.

Under the frame of this activity an evaluation of the in vitro cytotoxicity was made for the original and generic versions rectal cancer neoadjuvant oxaliplatin and carboplatin on tumor cell lines DLD-1, HT and A The inhibitory effect the original oxaliplatin drug against colon cell lines was not significantly higher, but the effect on the hpv in mouth and throat CD positive cells isolated from the whole cell population was superior in the K-ras mutant DLD-1 cell line after 24 hours exposure.

The chemoresistance towards the platinum-based drugs was higher in the HT Braf-mutant cell line, the toxicity being below of that for DLD We found morphologic evidences such prevailing membrane structure alteration, chromatin condensation in the tumor cells treated with original drugs.

The phenomenon studied in vitro can be extrapolated to the clinics to anticipate the drugs efficacy, and to predict at some extent the hypersensibility reactions and the adverse effects of the drugs. The experiment was conducted in vitro on human colorectal cell lines, the toxicity was evaluated with colorimetric methods, and the simultaneous expression of the molecules was quantified using rectal cancer neoadjuvant flow cytometry. The transcription factors were correlated rectal cancer neoadjuvant the expression of the molecules implicated in VEGF signaling.

Overall, the studied compounds exhibit antiproliferative effect against the tumor cells in vitro, they influenced the NF-kβ signaling and the angiogenesis.

Programul national de oncologie

In addition, rectal cancer neoadjuvant evaluated alchylphosphocholine ligands of metal compounds, highlighting their antimicrobial and antitumor activity. Pin on tratamente, remedii, retete The activities completed during the phases I to IV of the project October 3rd December 10th were: 4.

Assesment of immunomodulatory effects of chemotheray in colorectal carcinoma, objective completed through two major activities: The evaluation of peripheral blood mononuclear cell rectal cancer neoadjuvant proportion and activation status following the treatment with standard platinum drugs, and In vitro evaluation of immune system modulation and cytotoxicity of novel synthesized metal-based compounds with prodrug potential in colon carcinoma 4.

Correlation of in vitro and ex vivo outcome resulted from neoadjuvant clinical treatment in colorectal cancer in order to locate potential prediction factors, accomplished by the activity: Prediction factors elucidation in colorectal cancer pacients, and the treatment follow-up. Evaluation of the biochemical features of platinum-based drugs effect evaluation in tumoral stem cells.

Colorectal cancer neoadjuvant therapy, Papilloma alla vescica nell uomo

In vitro assessment of platinum-drug effects on stem cells isolated from human tumoral cell lines and human tissues. Correlations between in vitro testing and clinical results. The study of platinum rectal cancer neoadjuvant palladium compounds effect on genetically mutant tumor tissues.

rectal cancer neoadjuvant

Ex-vivo evaluation of platinum-based compounds effect on stem cells from colorectal tumors and colorectal cell lines. Contact person: Dr.

Neoadjuvant treatment's tumor response in rectal cancer - Jurnalul de Chisturi de helmint Scripcariu Universitatea de Medicină şi Farmacie ,Gr.